Biochemist I/II - Recombinant Protein Manufacturing
Job Title
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Biochemist I/II - Recombinant Protein Manufacturing
Location(s)
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San Diego - BioLegend
About Us
Revvity is a developer and provider of end-to-end solutions designed to help scientists, researchers, and clinicians solve the world’s greatest health challenges. We pair the enthusiasm of an industry disruptor with the experience of a longtime leader. Our team of 11,000+ colleagues from around the globe are vital to our success and the reason we’re able to push boundaries in pursuit of better human health.
Find your future at Revvity
This position is responsible for manufacturing cutting edge recombinant protein research products in Molecular Cellular Biology (MCBio) group. The recombinant manufacturing biochemist will be involved in different stages of production of various recombinant protein products, which includes recombinant protein expression and purification.
The duties include preparing buffer solutions and cell culture media, performing bacterial cultures, affinity purification of recombinant proteins, and performing DNA isolation according to standard operating procedures. Independence in production of high complex bacterial projects, understanding and troubleshoot technical issues, internal audit, and document revision training. Additionally, biochemist will be responsible in project support which may include transferring the products to other groups for testing, preparing product/project related documentation, and maintaining the inventory and record for recombinant protein products.
Essential Functions
Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.
- Prepare various buffers for experiments, including cell culture media.
- Perform transformation and culture of bacterial cells (1-50 liters)
- Process the culture supernatant/cell pellet/inclusion body, followed by extensive processing of the supernatant/cell pellet/inclusion body or diafiltration to extract or refold raw protein bulk.
- Perform purifying recombinant protein by means of affinity tag
- ...
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